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For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol

  • Clean-up and concentrate total RNA in minutes
  • No phenol or chloroform extractions
  • Clean-up RNA from contaminants including enzymes, RNases and nucleotides 
  • Rapid spin-column protocol 
  • Also available in a 96-well format for high throughput applications
  • Purified RNA is fully compatible with all downstream applications

Item

Cat. No.

Kit Size

RNA Clean-Up and Concentration Kit

AV1004

50 preps

 

Accuvis’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The kit can concentrate as much as 35 µg of RNA to as little as picograms of RNA. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).  The RNA is preferentially purified from other reaction components such as proteins, RNases and nucleotides without the use of phenol or chloroform.

Purification is based on spin column chromatography using Accuvis’s proprietary resin as the separation matrix. Briefly, the RNA samples or reactions containing RNA are mixed with Binding Solution, ethanol is added and the RNA is bound to Accuvis's column (BIND).  Under these conditions only the RNA will bind to Accuvis's resin while most of the contaminating proteins and nucleotides are removed in the flowthrough.  The bound RNA is then washed to remove any remaining impurities (WASH).  Lastly, the purified total RNA is eluted into 20 - 50 µL of the provided Elution Buffer or water (ELUTE).  Please see procedure flowchart to the right.
The purified RNA is of the highest integrity, and can be used in a number of downstream applications. Accuvis’s RNA Clean-Up and Concentration Kit purifies RNA with minimal amounts of DNA contamination. An optional protocol is provided for maximum removal of residual DNA that may affect sensitive downstream applications such quantitative PCR.
This kit is also available in a 96-well format for high-throughput RNA clean-up and concentration.  Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
  • Efficient clean-up from various enzymatic reactions - RNA clean-up from upstream enzymatic applications such as labeling, DNase treatment and in vitro transcription. Remove proteins, RNases, DNases, nucleotides, etc.
  • Clean-up and concentration of RNA isolated by different methods - RNA that has been isolated using various methods, including phenol-chloroform extractions or alcohol precipitations, can be processed with this kit
  • Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes, while the 96-well plates can be processed in 30 minutes
  • No phenol:chloroform extractions - Accuvis’s RNA Clean-Up and Concentration Kit purifies RNA without the use of harmful chemicals such as phenol or chloroform.
  • Isolate all small RNA molecules - All small RNA species can be isolated including microRNA, siRNA, tRNA, and 5S rRNA
  • Recovered RNA is suitable for downstream applications - Purified RNA can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, and expression array analysis